cp-6 · image #25

This report summarises image #25 from well A01, field 04, showing the Mito channel. The well was treated with gabapentin-enacarbil, a compound with the annotated target gene CACNB4.

The provided quality metrics are for the corresponding DNA channel in this field, not the Mito channel itself. Based on these metrics, the DNA image is severely out of focus (ImageQuality_FocusScore_DNA = 0.00496). The image has low overall intensity (ImageQuality_MeanIntensity_DNA = 0.045) but does contain saturated pixels (ImageQuality_MaxIntensity_DNA = 1.0), although they constitute a very small portion of the image (ImageQuality_PercentMaximal_DNA = 0.003%).

Despite the poor focus, CellProfiler identified a high number of objects (Count_Nuclei = 511.0). The segmentation threshold was set at Threshold_FinalThreshold_Nuclei = 0.0473, which is close to the image's mean intensity. The high object count in a poorly focused image suggests potential oversegmentation of blurry nuclear objects.

The primary QC flag is the extremely poor focus score for the DNA channel. This critical failure likely renders any downstream measurements from this field, including those from the Mito channel, unreliable. The image should be flagged for exclusion from analysis.