cp-5 · image #59

This report summarises the RNA channel (ch3, SYTO 14) from field 08 of well A01. The well was treated with gabapentin-enacarbil, a compound annotated as targeting the gene CACNB4.

Image quality metrics for the RNA channel were not provided. The metrics for the corresponding DNA channel show a low focus score (ImageQuality_FocusScore_DNA = 0.1107) and no evidence of saturation (ImageQuality_PercentMaximal_DNA = 8.57e-05). The mean intensity of the DNA channel was low (ImageQuality_MeanIntensity_DNA = 0.0333).

CellProfiler identified a total of 104 nuclei in this field (Count_Nuclei = 104.0). The segmentation threshold for these nuclei was set at Threshold_FinalThreshold_Nuclei = 0.0453. No cell-level or RNA-specific measurements were provided to assess the effect of the perturbation.

A significant QC flag is raised: all provided image quality metrics are for the DNA channel, not the RNA channel under review. This data mismatch prevents a direct quality assessment of this specific image. The low focus score in the DNA channel may indicate a broader focus issue with the image acquisition field.