cp-4 · image #5

This summary covers image 5 from well A01, field 01. The image captures the DNA channel (ch5), stained with Hoechst 33342.

The image quality appears poor. The focus is low (ImageQuality_FocusScore_DNA = 0.087). The image is dim, with a low mean intensity (ImageQuality_MeanIntensity_DNA = 0.0196). There are no signs of pixel saturation, with a very low percentage of maximal pixels (ImageQuality_PercentMaximal_DNA = 8.57e-05).

CellProfiler identified 138 nuclei (Count_Nuclei = 138.0) using a final threshold of 0.050 (Threshold_FinalThreshold_Nuclei = 0.05025). The overall intensity distribution is narrow, with a median intensity of 0.0094 (ImageQuality_MedianIntensity_DNA = 0.0093995575) and a maximum of 0.62 (ImageQuality_MaxIntensity_DNA = 0.620676).

A QC flag should be raised for poor focus. The low focus score (ImageQuality_FocusScore_DNA = 0.087) indicates a blurry image, which will compromise the accuracy of nuclear segmentation and downstream measurements. The low overall intensity may also be a concern.